isolation and characterization of diazinon degrading bacteria from contaminated agriculture soils

background: organic agricultural pesticides are so stable in ecosystems and biosphere. these compounds affect soil conditions and agricultural products. the aim of this study was isolation of diazinon-degrading bacteria from kerman pistachio orchards, southeastern iran. methods: diazinon-degrading bacteria were enriched in bushnell-hass medium. identification and sequencing of prevalent degrading strains was performed using pcr based on amplifying of 16s rdna. results: the soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. three diazinon-degrading bacteria strains belong to: pseudomonas putida strain d3, p. fluorescens strain d1 and achromobacter piechaudii strain d8. the best degrading strain (d1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. the results of gas-chromatography (gc) confirmed the decomposition of organic pesticide by degrading-bacteria. conclusion: the results of gas-chromatography (gc) confirmed the decomposition of organic pesticide by degrading-bacteria. using these strains and other biological reclamation methods we can eliminate bio-environmental problems.